- #MESTRENOVA UNKNOWN FORMAT OF FILE TROUBLESHOOT PDF#
- #MESTRENOVA UNKNOWN FORMAT OF FILE TROUBLESHOOT FULL#
You must include a hard copy of all NMR spectra for each experiment with your laboratory report.ģ) Preparing and submitting samples for GC-MS analysis All GC-MS analysis in CHEM 344 is performed upon dilute solutions except for the E1 andĮ2 product samples.
#MESTRENOVA UNKNOWN FORMAT OF FILE TROUBLESHOOT PDF#
Iv) Make sure that all signals that need specific coupling information are peak picked in Hz.ĭ) Save the MNova spectrum in worked-up format and as a pdf for easy printing. iii) Make sure all signals related to the reagents, products, byproducts, or solvents are ii) Make sure that the baseline is relatively flat and correctly phased. I) Make sure the x-axis scale is set to display all important signals are shown in the spectrum. How to work up the spectrum will be provided for the early experiments. The directions will change for each experiment, soīe sure to consult the example stock spectrum and specific directions each time. Instructions for obtaining MestReNova are on the course website.Ĭ) Work up all spectra by following the directions on the course website for each experiment and in the same manner as the stock spectrum. In the course are in the frequency-domain, showing intensity vs. time) to the frequency domain (intensity vs. MestReNova will convert the fid from the time-domain (signal intensity vs. They have no fileī) Open the fid file in MestReNova. The five files are fid, log, procpar, sampleinfo, and text. Detailed instructions are available on the course website. Is available for analysis for each experiment.Ī) Obtain a folder of 5 key files for your NMR data. If you fail to obtain data, you will not be penalized, a stock spectrum Your lab report - submitting data as your own that you did not obtain is scientific fraud andĪcademic misconduct. You must submit your own spectral data with You will need to convert the raw data to a useable spectrum to beĪnalyzed and interpreted for each lab report. All 1H-NMR, 13C-NMR, 19F-NMR, and 31P-NMR data will be provided as the raw free. iii) Always include an unknown number or letter when applicable.Ģ) Obtaining NMR spectral data for analysis. ii) Write your first name and last initials on the NMR sample submission sheet. I) Always use the next available numbered slot in the rack do not skip spaces. h) Once the NMR tube is placed in the spin collar to the correct depth, place the tube in the sample G) Use the depth gauge to ensure that the sample is in the proper position. This is unsafe and can cause the tube to snap. Always push the tube into the spin collar from as close to the spin collar as possible. Dispose of the glass pipettes in the glass waste bin.į) Take a spinner from the rack and gently push the NMR tube into the spin collar. The sample should fill the NMR tube to a volume of to full.Į) Once the solution has been transferred, cap the NMR tube and take it to the metal sample rack designated for your laboratory section. d) Mix the two compounds to give a homogeneous solution and then transfer the sample into aĬlean NMR tube via Pasteur pipette. 1.5 mL (~1 pipette load) of CDCl3 to the vial. c) With another clean Pasteur pipette, add approx.
#MESTRENOVA UNKNOWN FORMAT OF FILE TROUBLESHOOT FULL#
Prepare and submit a sample for 1H-NMR analysis:Ī) For liquids or oils, use a clean Pasteur pipette to transfer ~4 drops of the liquid into a clean sample vial.ī) For solids, add a liberal spatula-tip full of compound (40 - 50 mg) into a clean sample vial. Liquids, biphasic solutions, or solutions containing solids give poor quality NMR data. Spectroscopy Sample Submission and Data Analysis Informationġ) Preparing and submitting samples for 1H-NMR analysis All 1H-NMR analysis in CHEM 344 is performed upon dilute solutions in CDCl3.